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KMID : 0894520120160010009
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Development & Reproduction
2012 Volume.16 No. 1 p.9 ~ p.18
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Effects of 4-Nonlyphenol Exposure on P.olivaceus and S.schlegeli Vitellogenesis
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Jung Jee-Hyun
Shim Won-Joon
Bae Sun-Hae
Baeck Kyong-Lan
Han Chang-Hee
Kim Dae-Jung
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Abstract
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The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder (Paralichthys olivaceus) and rockfish (Sebastes schlegeli) exposed continuously at 10, 50 and 100ugl{-1} levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17 B(E_2) and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma E_2 concentrations increased significantly in two female fish species exposed to 100ugl{-1} of 4-NP over concentrations in control fish. Plasma T concentrations increased in P.olivaceus. Four days after exposure, the level of VTG mRNA expression increased in P.olivaceus and S. schlegeli exposed to 20ugl{-1} of 4-NP. In addition, plasma VTG protein expression was seen in P.olivaceus and S.schlegeli. In S.schlegeli and P.olivaceus exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature S.schlegeli were the most sensitive to 4-NP exposure in vitellogenesis.
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KEYWORD
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Vitellogenin, Paralichthys olivaceus, Sebastes schlegeli, 4-Nonylphenol, Steroidogenesis, Endocrine disrupter
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